Sony Pixel Power calrec Sony

August 07, 2018

09/08/2018

New antibody analysis accelerates rational vaccine design Scripps Research study illuminates humoral immune response to experimental vaccines.

August 07, 2018

A team at Scripps Research has come up with a faster way to analyze the outcome of experimental vaccines against HIV and other pathogens. Their new system lets scientists quickly assess the full spectrum of antibodies produced in an individual in response to a pathogen or vaccine and determine if these antibodies are likely to be effective against the pathogen.

We can now watch antibody responses evolve almost in real time, says Lars Hangartner, PhD, a Scripps Research associate professor and co-senior author of the study, which published today in the journal Immunity.

This can be applied to any pathogen, adds Andrew Ward, PhD, professor at Scripps Research and study co-senior author. Ward is also affiliated with the International AIDS Vaccine Initiative (IAVI) Neutralizing Antibody Center and Center for HIV/AIDS Vaccine Immunology and Immunogen Discovery (CHAVI-ID) at Scripps Research.

When a pathogen attacks, your immune system amasses an army of antibodies to fight back. Some of these antibodies will fail, but some may get close to beating a pathogen's defenses. Over time, the best antibodies will evolve to target a pathogen's vulnerable sites. If all goes well, antibodies will home in on those epitopes, neutralize the invader and provide lifelong immunity.

Vaccines work by tricking the body into producing neutralizing antibodies against a future invader. Testing vaccines tends to be a lengthy process, with an initial immunization and then a series of booster shots over weeks or months. The time it takes to analyze the trial data means scientists don't usually get to see if a vaccine is working until the experiment is over, making it especially hard to tweak treatment strategies in the face of emerging diseases.

With the new study, scientists finally have a way to get a nearly real-time picture of antibody evolution. The new technique builds on Scripps Research-led breakthroughs in immunology and an imaging technique called electron microscopy, which reveals the structures of antibodies bound to their target pathogens, such as HIV.

The researchers used rabbit blood samples collected at different stages of an HIV vaccine trial. The researchers purified the samples, breaking down the molecules until they could extract antibody fragments. They then mixed these antibody fragments with their targets viral proteins and imaged them in the electron microscope, revealing how the immune system was developing its attack on the pathogen.

After fine-tuning the biochemistry behind the formation of immune complexes of HIV Env trimers with antibody fragments, we were able to establish a very solid protocol allowing for semi-quantitative electron microscopy analysis, says study co-first author Matteo Bianchi, PhD, a former Scripps researcher now with the Institute of Medical Virology, University of Zurich.

This new method is based on images taken by a relatively low-tech method called negative stain imaging, which helped them spot promising, or more often than not, distracting, antibodies. At last, the researchers could quickly find out if a vaccine was pushing the immune system along the right path. And in cases where a vaccine wasn't working, the method could provide researchers with information as to how the vaccine could be improved.

You can change the direction of a study depending on the results from this method, says study co-first author Hannah Turner, a research assistant in the Ward lab. Never before have we been able to take a snapshot of our antibodies like this giving us a more complete picture of what is happening and how we can influence the vaccination process.

While the images were not high-resolution, the researchers were excited to report that this level of analysis which only takes days could become a routine test around the world. Most institutes have at least the technology and resources to do this level of analysis, says Ward.

For an even closer look at the antibodies, Ward used higher-tech cryo-electron microscopy to get high-resolution, 3-D images of the antibodies with their viral targets. These detailed snapshots revealed additional details that may help scientists improve future experimental HIV vaccines.

The researchers say their faster method could open the door to personalized design for vaccines. For example, this study included a high-responder rabbit that produced many effective antibodies, and a low-responder rabbit with a weak army of antibodies. Going forward, researchers could use this new method in human vaccine or therapeutic trials to quickly separate high responders from low responders early on, saving crucial time for patients and their doctors as they search for effective therapies and vaccines that provide herd immunity.

This is going to be part of every immunization project I do from now on, Ward says.

This method has the potential to change the pace at which we can develop vaccines, adds Hangartner.

Additional authors of the study, Rapid mapping of polyclonal antibody responses by electron microscopy, were co-first authors Matteo Bianchi and Hannah Turner of Scripps Research; Bartek Nogal, Christopher Cottrell, David Oyen, Matthias Pauthner, Raiza Bastidas and Rebecca Nedellec of Scripps Research; Laura McCoy of University College London; Ian Wilson of Scripps Research, the International AIDS Vaccine Initiative-Neutralizing Antibody Center and the Center for HIV/AIDS Vaccine Immunology and Immunogen Discovery; and Dennis Burton of Scripps Research, the International AIDS Vaccine Initiative-Neutralizing Antibody Center, the Center for HIV/AIDS Vaccine Immunology and Immunogen Discovery; and the Ragon Institute.

The study was supported by the N
LINK: https://www.scripps.edu/news-and-events/press-room/2018/20180807-antib...
See more stories from scripps

Most recent headlines

13/03/2025

FCC Chairman Carr Launches Massive Deregulation Initiative

WASHINGTON FCC Chairman Brendan Carr announced that the agency has launched a massive, new deregulatory initiative that could potentially subject virtually all ...

13/03/2025

SDVI Integrates Rally With Spectra Vail

SUNNYVALE, Calif. SDVI has announced that it has integrated its Rally media supply chain management platform with the Spectra Vail multi-cloud data management s...

13/03/2025

FCC Approves Gray's Acquisition of KXLT

ATLANTA Gray Media has announced that the Federal Communications Commission (FCC) has granted a waiver of its local ownership rules to permit Gray Media to acqu...

13/03/2025

NAB Show 2025 Exhibitor Insight: Blackmagic Design

TV Tech: What do you anticipate will be the most significant technology trends at the 2025 NAB Show?...

13/03/2025

Watch Student Naomi Soleils Folk Pop Performance on The Voice

Watch Student Naomi Soleils Folk Pop Performance on The Voice The songwriting major sang Stars by Grace Potter and the Nocturnals during the blind auditions. ...

12/03/2025

Como o Impacto Cultural e Financeiro da Indstria Musical Define Seu Sucesso em 2025

O Spotify acaba de lan ar o relat rio Loud & Clear deste ano, uma vis o transpar...

12/03/2025

Cmo el impacto cultural y financiero de la industria musical define su xito en 2025

Spotify acaba de presentar el informe Loud & Clear de este a o, una mirada trans...

12/03/2025

Nourish Mind, Body, and Soul This Ramadan With Spotify

Ramadan, a period of profound spiritual significance for Muslims worldwide, is a time for fasting, prayer, reflection, and community. Enrich your experience thi...

12/03/2025

How the Music Industry's Cultural and Financial Impact Define Its Success in 2025

Spotify has just unveiled this year's Loud & Clear report, a transparent loo...

12/03/2025

FAST now transcends back catalog content

More than 70% of FAST programming has been produced since 2010, according to new Gracenote report NEW YORK March 12, 2025 Gracenote, the content data busin...

12/03/2025

Viamedia Adopts ShowSeeker Pilot To Streamline Ad Workflows

GEONA, Nev. Independent digital and linear advertising rep firm Viamedia will adopt cloud-based ShowSeeker Pilot as its primary ad campaign and order management...

12/03/2025

Mediagenix Appoints Wael Yasin as Sales Director Central Europe

BRUSSELS Mediagenix has announced that Wael Yasin has joined the company as sales director Central Europe....

12/03/2025

Omdia: Global Online Consumer Expenditure to Hit $6.6 Trillion by 2029

LONDON A new study highlights opportunities for shoppable TV and the massive impact online consumer spending is having on the economy, with Omdia predicting tha...

12/03/2025

Comcast Technology Solutions Upgrades to Interra Systems BATON Version 9

CUPERTINO, Calif. Interra Systems has announced that Comcast Technology Solutions has integrated recent updates to BATON Version 9 into its operations....

12/03/2025

Nevion announces new 400G addition to its eMerge SDN media fabric offering

Nevion announces new 400G addition to its eMerge SDN media fabric offering Brie Clayton March 12, 2025 0 Comments High-capacity switch enhances existi...

12/03/2025

DaVinci Resolve Studio Delivers Cinematic Sound for Adam Bol

DaVinci Resolve Studio Delivers Cinematic Sound for Adam Bol Brie Clayton March 12, 2025 0 Comments Feature film relies on DaVinci Resolve Studio for ...

12/03/2025

SVT Leverages Ateliere Live to Pioneer 100% Software-Defined Production at Rally Sweden 2025

SVT Leverages Ateliere Live to Pioneer 100% Software-Defined Production at Rally...

12/03/2025

Berklee Awards Fenway Neighborhood Improvement Grant to Four Organizations

Berklee Awards Fenway Neighborhood Improvement Grant to Four Organizations A total of $17,000 will be distributed among the Boston-based nonprofits. By Madd...

12/03/2025

Offering a broad umbrella for IP standards

TVBEuropes Jenny Priestley sits down with new SMPTE president Richard Welsh to discuss his aims for the organisation going forward, its efforts to attract a you...

12/03/2025

Warner Bros Belgium takes a new approach to its post production workflows

The new process has significantly enhanced operational efficiencies, with automation freeing up creators to concentrate on higher value tasks By Matthew Corrig...

12/03/2025

Another VFX company suspends operations

Jellyfish Pictures, which has offices in London and Sheffield, said it has been battling hard in the face of strong headwinds over the last 12 months By Jenny ...

12/03/2025

Bitcentral to Showcase AI-Powered Innovations for Smarter...

Visit Booth #W2213 to Experience the Latest in AI-Driven Content Discovery and Workflow Automation Bitcentral, a leader in media workflow solutions, is set to ...

12/03/2025

Jeff Lilly Named WGN-TV Director Of Technology

CHICAGO Jeff Lilly has been named WGN-TV director of technology effective March 17, 2025, according to Ric Harris, WGN-TV vice president and general manager....

12/03/2025

Survey: Consumers Want More Shoppable TV Experiences

MOUNTAIN VIEW, Calif. A new study from LG Ad Solutions indicates that consumers want more features that would allow them to shop for products on the connected T...

12/03/2025

IP Showcase To Focus On IP, Broadcast Tech Convergence at 2025 NAB Show

BOTHELL, Wash. The Alliance for IP Media Solutions (AIMS), Advanced Media Workflow Association (AMWA) and the Video Services Forum (VSF) will once again present...

12/03/2025

Comcast Boosts Internet Speeds for More Than 20 Million Customers

PHILADELPHIA Comcast announced that it has upgraded Xfinity Internet speeds for more than 20 million customers for no additional cost....

12/03/2025

Create with Maxon: Cinema 4D Fundamentals Workshop - March 12-14

Create with Maxon: Cinema 4D Fundamentals Workshop - March 12-14 Brie Clayton March 11, 2025 0 Comments Makin' Waffles with Elly Wade During Marc...

12/03/2025

Ross Video Strengthens Cloud Leadership with Appointment of Aaron Tunnell

Ottawa, Canada - March 12, 2025 - Ross Video is pleased to announce the appointment of Aaron Tunnell as Business Development Director, Cloud Solutions, reinforc...

12/03/2025

VEON to release 4Q 2024 trading update on 20 March 2025

12 Mar 2025 VEON to release 4Q 2024 trading update on 20 March 2025 Dubai, 12 March 2025 - VEON Ltd. (NASDAQ: VEON), a global digital operator, today confirms ...

12/03/2025

Getting Set for the Winter Olympics: Inside SVT's Ongoing Software-Based Production Evolution

Getting set for the Winter Olympics: Inside SVT's ongoing software-based pro...

12/03/2025

Seamless SMPTE 2110: A Discussion on Making the Move to IP Less Painful

Seamless SMPTE 2110: A Discussion on Making the Move to IP Less Painful Leaders from Netflix, Monumental Sports & Entertainment, LinkedI, and Megapixel address ...

12/03/2025

Opening Doors of Perception: Meta's Ajit Ninan on Rethinking AR/MR Perceptual Displays

Opening Doors of Perception: Meta's Ajit Ninan on Rethinking AR/MR Perceptua...

12/03/2025

Sky Sports Unveils Exciting Plans for 2025 Formula 1 Coverage

With the 2025 Formula 1 season set to begin in Melbourne on March 16, Sky Sports is gearing up to deliver its most comprehensive F1 coverage yet. Following a th...

12/03/2025

Unicanal and Trece TV revolutionize Digital TV in Paraguay with Rohde & Schwarz transmission solutions

Unicanal and Trece TV revolutionize Digital TV in Paraguay with Rohde & Schwarz ...

12/03/2025

Honoring Service, Celebrating Sport: Rohde & Schwarz UK Sponsors NavyFit Rugby Double-Header

Honoring Service, Celebrating Sport: Rohde & Schwarz UK Sponsors NavyFit Rugby D...

12/03/2025

FOR-A America Introduces Top of the Line HANABI Series Switcher at NAB 2025 Exhibition

New HVS-Q12 Represents a Quantum Leap in Switcher Design...

12/03/2025

Telespazio and ABS Expand Partnership to Deliver Tailored C-Band Connectivity Solutions for the Brazilian Market

Telespazio and ABS Expand Partnership to Deliver Tailored C-Band Connectivity So...

12/03/2025

Congratulations to all our clients who have achieved TAG certification through our independent validation

ABC conducts independent audits across the TAG programs. In the March 2025 TAG s...

12/03/2025

Moonlight: Thales Alenia Space to develop the space segment of the navigation system orbiting around the Moon

Facebook Twitter LinkedIn Moonlight: ESA program to create a satellite con...

12/03/2025

Thales among the world's top 100 most innovative companies for the 12th year, according to Clarivate

Facebook Twitter LinkedIn In 2025, Thales ranks among the top 0.01% of the...

12/03/2025

CCPC sponsored TV series The Complaints Bureau returns to RT One

Consumer show back following hugely successful first series 10 March 2025 Following a hugely successful first series, The Complaints Bureau returns to RT ...

11/03/2025

Give Me the Backstory: Get to Know Amel Guellaty, the Writer-Director of Where the Wind Comes From

By Lucy Spicer One of the most exciting things about the Sundance Film Festival...

11/03/2025

Salsa's Rhythmic Revival: A New Generation Discovers the Genre's Timeless Appeal

Salsa is making a comeback, captivating new listeners with its infectious energy...

11/03/2025

Canada's Own Rupi Kaur Curates the Latest Edition of Local Spot, Revealing the Songs and Artists That Shaped Her

For many people, music can serve as a reflection of their roots and upbringing. ...

11/03/2025

ST Engineering iDirect's Public Safety Solution Wins MSUA Satellite Mobile Innovation Award 2025

The solution delivers reliable, scalable and secure connectivity for critical pu...

11/03/2025

Weigel Broadcasting Taps Harmonic for Cloud-Based Playout-to-Delivery

SAN JOSE, Calif. Harmonic has announced that Weigel Broadcasting has deployed Harmonics VOS Media Software, which offers playout-to-delivery capabilities, inclu...

11/03/2025

Wheatstone To Unveil Linux Audio Driver At 2025 NAB Show

NEW BERN, N.C. Wheatstone will introduce a Linux audio driver for its WheatNet IP audio network during the 2025 NAB Show, April 5-9, at the Las Vegas Convention...

11/03/2025

Study: Women's Sports Are a Big Win for Advertisers

NEW YORK A new study finds that as TV viewership for women's sports surged by 131% in 2024, the programming also saw a 56% year-over-year increase in ad im...

11/03/2025

Al Sharqiya prepares for Ultra HD with FOR-A

Powerful switcher for news studio FOR-A, a cutting-edge video broadcast technology company backed by more than 50 years experience, has installed its HVS-1200 ...